Zeiss Light Sheet Z1

Our microscopy facility features cutting-edge light sheet fluorescence microscopes from Zeiss. Our goal is to advance the research of the Boston community by offering competitive pricing and expertise.

Application Forms

Zeiss LSM 880

Instrument Snapshot

Light sheet fluorescence microscopes are known for being easy to use and best to use for imaging thin or whole animal samples and long-term imaging of live samples.

Due to the thin, but powerful, sheet of light that this scope has, samples are easily penetrated, providing clear, bright images. Lighting a small section of the sample at once, the Zeiss Light Sheet Z1 also minimizes phototoxicity and photobleaching.

Arivis Vision4D software enables image analysis and rendering.

Reach out to our core facility in Boston with any questions at cils-core@northeastern.edu.



Laser line: 405, 488, 561, 638 nm


Objective: 5×, 10×-water, 20×-CLARITY


CO2/temperature control


Large scale 3D tissue imaging


arivis Vision4D software

Sample/Previous Images

Spinal Cord – Axolotl

Instrument: Zeiss Lightsheet Z1
Stained with 5-ethynyl uridine (EU), RNA synthesizing cells (cyan) and cell nuclei (magenta)
Credit: Jackson Griffiths/Monaghan Lab

African Clawed Frog (Xenopus Laevis) Embryo

Instrument: Zeiss Lightsheet Z1
Expressing green flourescent protein
Credit: Ashwin Lokapally/Sive Lab

Axolotl Limb Regeneration

Instrument: Zeiss Lightsheet Z1
Genetically encoded cell cycle reporter. Magenta: G1 phase cells. Yellow: S/G2/M phase cells
Credit: Eun Jeon/Monaghan Lab

Rat Brain Tissue

Treated with a retroviral vector to stain neurons
Instrument: Zeiss Lightsheet Z1
Credit: Shansky Lab


Internal NU


External Academia






Additional Resources

Carl Zeiss Microscopy Online Campus

Education in Microscopy and Digital Imaging

Light-Sheet Fluorescence Microscopy Overview

ZEISS Lightsheet Z.1 Sample Preparation

ZEISS Lightsheet Z.1 Quick Guide:

Imaging with Water or Clearing Immersion

ZEISS Lightsheet Z.1 Quick Guide:

Tile Scanning

Lightsheet Z.1 Operating Manual

Detailed guide to Lightsheet Z.1

Other Instruments

Zeiss LSM 800 (Inverted)

NU user: $30/hr; Academia: $50/hr; Industry: $70/hr

Laser line: 405, 488, 561, 640nm

Objective: 10×20×40×63×

Fixed cell/tissue imaging

Imaging Tiling


Zeiss Axiozoom V16 (Upright)

NU user: $20/hr; Academia: $30/hr; Industry: $40/hr

Continuous zoom with high NA

Large field of view

Apotome.2 Structured Illumination Module

In vivo and model organism imaging

Zeiss LSM 880 NLO (Upright)

NU user: $50/hr; Academia: $70/hr; Industry: $90/hr

Laser line: 405, 458, 488, 514, 561, 594, 640 nm

NIR Laser: tunable line (680-1300 nm); fixed line -1045 nm

Objective: 10×20×20×, 40×20×-CLARITY

Multi-photon imaging

Superresolution (Airyscan)

Spectral Unmixing

Prerequisite: Bioraft Laser Safety Training Part I and II

Our facility

Interdisciplinary Science and Engineering Complex

ISEC 080, 805 Columbus Ave, Boston, MA, 02115