Beckman Coulter CytoFLEX SRT Cell Sorter

Our flow cytometry facility provides services for cell sorting and analyzing needs. Our goal is to advance the research of the Boston community by offering competitive pricing and expertise.

Application Forms

Internal

External

Zeiss LSM 880

Instrument Snapshot

Our Beckman Coulter CytoFLEX SRT cell sorter offers four different laser lines for detecting a wide variety of cells. This instrument is fast, less than 30 minutes between setup and sample sorting.

The cell sorter features multiple sorting modes: 4-way Sorting; enrichment or purity or single, ability to catch aborts. After cells are sorted, there’s a high recovery rate, enabling further experimentation to be conducted.

Reach out to our core facility in Boston with any questions at cils-core@northeastern.edu.

Features

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4 laser lines: 405, 488, 561, 638

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15 color detection

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100nm nozzle

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Sort into 1-4 tubes, slides, or into various well plates

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Mixed Mode sorting

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Violet side scatter for small particle detection

Rates

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Internal NU

$65/hr

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External Academia

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Industry

Additional Resources

CytoFLEX SRT - Instructions for Use

Part 1

CytoFLEX SRT - Instructions for Use

Part 2

Available lasers and filters

Other Instruments

Zeiss LSM 800 (Inverted)

NU user: $30/hr; Academia: $50/hr; Industry: $70/hr

Laser line: 405, 488, 561, 640nm

Objective: 5×, 10×, 20×, 40×, 63×

Fixed cell/tissue imaging

Imaging Tiling

Zeiss LSM 880 NLO (Upright)

NU user: $50/hr; Academia: $70/hr; Industry: $90/hr

Laser line: 405, 458, 488, 514, 561, 594, 640 nm

NIR Laser: tunable line (680-1300 nm); fixed line -1045 nm

Objective: 10×, 20×, 20×, 40×, 20×-CLARITY

Multi-photon imaging, Spectral Unmixing

Superresolution (Airyscan )

Prerequisite: Bioraft Laser Safety Training Part I and II

Beckman Coulter CytoFLEX S Flow Cytometer

NU user: $40/hr

4 laser lines: 405, 488, 561, 638

13 color detection

Can load tube or various well plates for analysis

Violet side scatter for small particle detection

Able to interface experiment design into SRT sorter

Get in Touch

617.373.4565
cils@northeastern.edu
cils-core@northeastern.edu

Our facility

Interdisciplinary Science and Engineering Complex

ISEC 080, 805 Columbus Ave, Boston, MA, 02115

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