CORE FACILITIES
We are now offering 70% off rate for nightly (6PM-9AM) and weekend/holiday usage to encourage schedule staggering during the COVID-19 Pandemic
Get Started With a Free Test Session
Microscopy Core Facility
ZEISS LSM 880 NLO (upright)
NU user: $55/hr; Academia: $75/hr; Industry: $95/hr
Laser line: 405, 458, 488, 514, 561, 594, 640 nm
NIR Laser: tunable line (680-1300 nm); fixed line -1045 nm
Objective: 10×, 20×, 20×, 40×, 20×-CLARITY
Multi-photon imaging
Superresolution (Airyscan)
Spectral Unmixing
Prerequisite: Bioraft Laser Safety Training Part I and II
ZEISS LIGHTSHEET Z1
NU user: $55/hr; Academia: $75/hr; Industry: $95/hr
Laser line: 405, 488, 561, 638 nm
Objective: 5×, 10×-water, 20×-CLARITY
CO2/temperature control,
Large scale 3D tissue imaging
arivis Vision4D software
ZEISS AXIO OBSERVER (DEMO, inverted)
NU user: free; Academia: $30/hr; Industry: $50/hr
Epifluorescence: DAPI, GFP, DsRed, Cy 5
Objective: 10×, 20×, 40×, 63×, 100×
Immunofluorescence labeled tissue or cell culture imaging
Preclinical Imaging Core Facility
BRUKER BIOSPEC 3T MRI
NU user: $30/hr; Academia: $150/hr; Industry: $200/hr
Resolution below 200 μm
Automated animal positioning
Multimodal rat/mouse cradle
Two body volume coils for rat/mouse
SAII 1030 monitoring/gating module
Localized MR Spectroscopy
Prerequisite: Bioraft High Magnetic Fields Safety Training
VisualSonics Vevo F2 Ultrasound
NU user: $60/hr; Academia: $110/hr; Industry: $140/hr
Linear Probe: 57-25 MHz for mouse cardiovascular, abdominal, reproductive; embryology; small rat vascular
Linear Probe: 29-15 MHz for rat cardiac & abdominal (<250g)
Phased Probe: 8-4 MHz for large animal cardiology & abdominal
Prerequisite: Bioraft Biosafety Training
Flow Cytometry Core Facility
BECKMAN COULTER CYTOFLEX SRT CELL SORTER
- NU user: $65/hr; Academia: $110/hr; Industry: $165/hr
- 4 laser lines: 405, 488, 561, 638 nm
- 15 color detection
- 100 um nozzle
- Sort into 1-4 tubes, slides, or into various well plates
- Mixed Mode sorting
- Violet side scatter channel for small particle detection
BECKMAN COULTER CYTOFLEX S FLOW CYTOMETER
- NU user: $40/hr; Academia: $70/hr; Industry: $130/hr
- 4 laser lines: 405, 488, 561, 638 nm
- 13 color detection
- Can load tube or various well plates for analysis
- Violet side scatter channel for small particle detection
- Able to interface experiment design into SRT sorter
Additional Instruments
NanoTemper Monolith NT.Automated
NU user: free; External: Please reach out to Professor Jared Auclair
Step-by-Step Guide for New NU User and Application Form
Fast Kd measurement: ~10 min
Low sample volume
Wide Kd range from nM to mM range
No need for immobilization
Measurements in complex environments
Rates
| Instrument | Internal NU (hourly) | External Academic (hourly) | External Industry (hourly) |
|---|---|---|---|
| Zeiss LSM 800 | $35 | $55 | $75 |
| Zeiss LSM 880 | $55 | $75 | $95 |
| Zeiss LSM 880 NLO | $55 | $75 | $95 |
| Zeiss Lightsheet Z1 | $55 | $75 | $95 |
| Zeiss Axiozoom V16 | $25 | $35 | $50 |
| Zeiss Axio Observer (Demo) | Free | $30 | $50 |
| Bruker BioSpec 3T MRI | $30 | $150 | $200 |
| Visualsonics Vevo F2 Ultrasound | $60 | $110 | $140 |
| Beckman Coulter CytoFlex SRT Cell Sorter | $65 | $110 | $165 |
| Beckman Coulter CytoFlex S Flow Cytometer | $40 | $70 | $130 |
**Rates listed above are for unassisted session. Hourly rate for training and assisted session is base rate+$30
Off-hours usage: 70% off from 6pm-9am on weekdays, full day on weekends/holidays
We are studying the dynamics of matrix assembly by human fibroblasts as they are producing tissue. The CILS facilities, particularly the confocal microscope with live imaging capabilities, has been indispensable to us. The team there has been wholly supportive and has enabled us to procure data we only dreamed about having previously. In fact, less than a week ago, I put fresh images from CILS directly into an NIH R01 proposal just in time for submission. It was the key missing piece of preliminary data I needed. So, thank you, CILS!
Our facility
Interdisciplinary Science and Engineering Complex
ISEC 080, 805 Columbus Ave, Boston, MA, 02115