CORE FACILITIES

NU Users: 70% off rate for nightly (6PM-9AM) and weekend/holiday usage to encourage schedule staggering

Microscopy

Flow cytometry Preclinical Imaging

Get Started With a Free Test Session

Instrument(s) of Interest

5 + 2 =

How to Get Started

Microscopy Core Facility

ZEISS LSM 800 (Inverted)

ZEISS LSM 880 NLO (upright)

ZEISS AXIOZOOM V16 (upright)

  • NU user: $25/hr; Academia: $35/hr; Industry: $50/hr
  • Two objectives with changeable magnification
  • Max magnification and WD: 0.5×/10 cm; 2.3×/1cm
  • Epifluorescence: DAPI, GFP, BODIPY, PI, RFP, Cy5, #71
  • Apotome.2 Structured Illumination Module
  • In vivo and model organism imaging

ZEISS LSM 900 (inverted)

ZEISS LSM 880 (Inverted)

ZEISS LIGHTSHEET Z1

  • NU user: $55/hr; Academia: $75/hr; Industry: $95/hr
  • Laser line: 405, 488, 561, 638 nm
  • Objective (Free Working Distance/mm): 2.5×(8.7), 5×(18.5), 10×W(3.7)20×-CLARITY(5.6)
  • CO2/temperature control,
  • Large scale 3D tissue imaging
  • arivis Vision4D software

ZEISS AXIO OBSERVER (DEMO, inverted)

Bruker Nanowizard 4 xp (AFM)

  • COMING SOON (First Half of  2024)
  • Coupled with Zeiss LSM 900
  • NU user: $45/hr; Academia: $65/hr; Industry: $85/hr
  • Collrelative microsscopy: integrate with confocal
  • Large scan field of 100 × 100 × 15 µm3and atomic lattice resolution

Flow Cytometry Core Facility

BECKMAN COULTER CYTOFLEX SRT CELL SORTER

  • NU user: $65/hr; Academia: $110/hr; Industry: $165/hr
  • 4 laser lines: 405, 488, 561, 638 nm
  • 15 color detection
  • 100 um nozzle
  • Sort into 1-4 tubes, slides, or into various well plates
  • Mixed Mode sorting
  • Violet side scatter channel for small particle detection

BECKMAN COULTER CYTOFLEX S FLOW CYTOMETER

  • NU user: $40/hr; Academia: $70/hr; Industry: $130/hr
  • 4 laser lines: 405, 488, 561, 638 nm
  • 13 color detection
  • Can load tube or various well plates for analysis
  • Violet side scatter channel for small particle detection
  • Able to interface experiment design into SRT sorter

Preclinical Imaging Core Facility

BRUKER BIOSPEC 3T MRI

VisualSonics Vevo F2 Ultrasound

  • NU user: $60/hr; Academia: $110/hr; Industry: $140/hr
  • Linear Probe: 57-25 MHz for mouse cardiovascular, abdominal, reproductive; embryology; small rat vascular

  • Linear Probe: 29-15 MHz for rat cardiac & abdominal (<250g)

  • Phased Probe: 8-4 MHz for large animal cardiology & abdominal

  • Prerequisite: SciShield Biosafety Training

 

Photon etc IR vivo NIR-II IMager

  • NU user: free;
  • Training: Contact Professor Allison Dennis
  • Illuminaton: Laser at 670, 760, 808 nm
  • Emission Range: 900-1600 nm
  • Lens: 50mm f/1.4
  • FOV: 5 x 4 to 15.6 x 12.5 cm
  • Heated Stage

PRECISION X-RAD 320 Irradiator/Imager

  • NU User: $50/h; Academia: $80/h; Industry: $110/h
  • Real-time specimen viewing
  • Co-registration of bioluminescence and x-ray images
  • Radiation dosage control through programmable shelf
  • Beam conditioning filters
  • Prerequisite: Scishield X-Ray Safety Training

Additional Instruments

NanoTemper Monolith NT.Automated

NU user: free

  • Step-by-Step Guide for New NU User
  • Fast Kd measurement:  ~10 min
  • Low sample volume
  • Wide Kd range from nM to mM range
  • No need for immobilization
  • Measurements in complex environments

BIO-RAD ChemiDoc™ MP

NU user: $50/lab/quarter; External: $100/quarter

Hourly Rates

<      
Instrument NU User Academia Industry
Zeiss LSM 800 $35 $55 $75
Zeiss LSM 880 $55 $75 $95
Zeiss LSM 880 NLO $55 $75 $95
Zeiss Lightsheet Z1 $55 $75 $95
Zeiss Axiozoom V16 $25 $35 $50
Zeiss Axio Observer (Demo) Free $30 $50
Zeiss LSM 900 $45 $65 $85
Bruker NanoWizard 4 XP AFM $45 $65 $85
Beckman Coulter CytoFlex SRT Cell Sorter $65 $110 $165
Beckman Coulter CytoFlex S Flow Cytometer $40 $70 $130
Bruker BioSpec 3T MRI $30 $150 $200
Visualsonics Vevo F2 Ultrasound $60 $110 $140
Precision X-Rad 320 X-Ray Irradiator/Imager $50 $80 $110

Rates listed above are for unassisted session. Hourly rate for training and assisted session is base rate+$30

NU user off-hours usage: 70% off from 6pm-9am on weekdays, full day on weekends/holidays

Reviewed and approved by NURes Finance (Approval Letter).

We are studying the dynamics of matrix assembly by human fibroblasts as they are producing tissue. The CILS facilities, particularly the confocal microscope with live imaging capabilities, has been indispensable to us. The team there has been wholly supportive and has enabled us to procure data we only dreamed about having previously. In fact, less than a week ago, I put fresh images from CILS directly into an NIH R01 proposal just in time for submission. It was the key missing piece of preliminary data I needed. So, thank you, CILS!

Jeffery Ruberti

Professor, Bioengineering

Our facility

Interdisciplinary Science and Engineering Complex

ISEC 080, 805 Columbus Ave, Boston, MA, 02115