CORE FACILITIES

We are now offering 90% off rate for nightly (6PM-9AM) and weekend/holiday usage to encourage schedule staggering during the COVID-19 Pandemic

Microscopy

MRIFlow cytometry

Get Started With a Free Test Session

Instrument(s) of Interest

11 + 1 =

Microscopy Core Facility

ZEISS LSM 800 (Inverted)

NU user: $30/hr; Academia: $50/hr; Industry: $70/hr

Laser line: 405, 488, 561, 640 nm
Objective: 10×20×40×63×
Fixed cell/tissue imaging,
Image Tiling

ZEISS LSM 880 NLO (upright)

NU user: $50/hr; Academia: $70/hr; Industry: $90/hr

Laser line: 405, 458, 488, 514, 561, 594, 640 nm
NIR Laser: tunable line (680-1300 nm); fixed line -1045 nm
Objective: 10×20×20×, 40×20×-CLARITY
Multi-photon imaging
Superresolution (Airyscan)
Spectral Unmixing
Prerequisite: Bioraft Laser Safety Training Part I and II

ZEISS AXIOZOOM V16 (upright)

NU user: $20/hr; Academia: $30/hr; Industry: $40/hr

Continuous zoom with high NA
Large field of view
Apotome.2 Structured Illumination Module
In vivo and model organism imaging

ZEISS LSM 880 (Inverted)

NU user: $50/hr; Academia: $70/hr; Industry: $90/hr

Laser line: 405, 458, 488, 514, 561, 594, 640 nm
Objective: 10×20×40×40×63×
Cage incubator, CO2/O2 control,
Superresolution (Airyscan)
Spectral Unmixing

ZEISS LIGHTSHEET Z1

NU user: $50/hr; Academia: $70/hr; Industry: $90/hr

Laser line: 405, 488, 561, 638 nm
Objective: 5×, 10×-water20×-CLARITY
CO2/temperature control,
Large scale 3D tissue imaging
arivis Vision4D software

ZEISS AXIO OBSERVER (DEMO, inverted)

NU user: free

Epifluorescence: DAPI, GFP, DsRed, Cy 5
Objective: 10×, 20×, 40×, 63×, 100×
Immunofluorescence labeled tissue or cell culture imaging

MRI Core Facility

BRUKER BIOSPEC 3T MRI

NU user: $20+/hr; Academia: $150/hr; Industry: $200/hr

Automated animal positioning
Multimodal rat/mouse cradle
Two body volume coils for rat/mouse
SAII 1030 monitoring/gating module
Localized MR Spectroscopy
Prerequisite: Bioraft High Magnetic Fields Safety Training

Flow Cytometry Core Facility

BECKMAN COULTER CYTOFLEX SRT CELL SORTER

  • NU user: $65/hr
  • 4 laser lines: 405, 488, 561, 638
  • 15 color detection
  • 100 um nozzle
  • Sort into 1-4 tubes, slides, or into various well plates
  • Mixed Mode sorting
  • Violet side scatter for small particle detection

BECKMAN COULTER CYTOFLEX S FLOW CYTOMETER

  • NU user: $40/hr
  • 4 laser lines: 405, 488, 561, 638
  • 13 color detection
  • Can load tube or various well plates for analysis
  • Violet side scatter for small particle detection
  • Able to interface experiment design into SRT sorter

Additional Instruments

NanoTemper Monolith NT.Automated

NU user: free; External: Please reach out to Professor Jared Auclair

Step-by-Step Guide for New NU User and Application Form

Fast Kd measurement:  ~10 min
Low sample volume
Wide Kd range from nM to mM range
No need for immobilization
Measurements in complex environments

Rates

Instrument Internal NU (hourly) External Academic (hourly) External Industry (hourly)
Zeiss LSM 800 $30 $50 $70
Zeiss LSM 880 $50 $70 $90
Zeiss LSM 880 NLO $50 $70 $90
Zeiss Lightsheet Z1 $50 $70 $90
Zeiss Axiozoom V16 $20 $30 $40
Zeiss Axio Observer (Demo) Free $30 $50
Bruker BioSpec 3T MRI $20+ $150 $200
Beckman Coulter CytoFlex SRT Cell Sorter $65 $110 $165
Beckman Coulter CytoFlex S Flow Cytometer $40 $70 $130

**Rates listed above are for unassisted session. Hourly rate for training and assisted session is base rate+$30

Off-hours usage: 90% off from 6pm-9am on weekdays, full day on weekends/holidays

Extended imaging sessions: 30% off after the first three hours of imaging at full hourly price

We are studying the dynamics of matrix assembly by human fibroblasts as they are producing tissue. The CILS facilities, particularly the confocal microscope with live imaging capabilities, has been indispensable to us. The team there has been wholly supportive and has enabled us to procure data we only dreamed about having previously. In fact, less than a week ago, I put fresh images from CILS directly into an NIH R01 proposal just in time for submission. It was the key missing piece of preliminary data I needed. So, thank you, CILS!

Jeffery Ruberti

Professor, Bioengineering

Our facility

Interdisciplinary Science and Engineering Complex

ISEC 080, 805 Columbus Ave, Boston, MA, 02115