Featured Images
From the CILS Core FacilityMouse Limb Regeneration
Instrument: Zeiss Lightsheet Z1
Technique: FISH, FGF8 in yellow, SHH in magenta, and gremlin 1 in cyan
Credit: Evan Mun/Monaghan Lab
Axolotl Limb Regeneration
Instrument: Zeiss LSM 880
Technique: FISH – Uromodulin(yellow), keratin 5 (cyan)-, Wnt5a (magenta)
Credit: Alex Lovely/Monaghan Lab
Brain Microvascular Network
Instrument: Zeiss LSM 800
Brain endothelial cells: dTomato (red). Pericytes: NG-2 (purple). Astrocytes: GFAP (green)
Credit: Max Winkelman/Dai Lab
African Clawed Frog (Xenopus Laevis) Embryo
Instrument: Zeiss Lightsheet Z1
Expressing green flourescent protein
Credit: Ashwin Lokapally/Sive Lab
Axolotl Limb Regeneration
Instrument: Zeiss Lightsheet Z1
Genetically encoded cell cycle reporter. Magenta: G1 phase cells. Yellow: S/G2/M phase cells
Credit: Eun Jeon/Monaghan Lab
Axolotl Limb Regeneration
Instrument: Zeiss Lightsheet Z1
Technique: FISH for three genes FGF8 (green), prrx1 (blue), and SHH (red)
Credit: Alex Lovely/Monaghan Lab
Shipworm Tissue in the Digestive Glands
Instrument: Zeiss LSM 880
Distribution of GH6 domain of a cellulase produced by intracellular bacterial symbiont
Credit: Marvin Altamia/Distel Lab
Spinal Cord – Axolotl
Instrument: Zeiss Lightsheet Z1
Stained with 5-ethynyl uridine (EU), RNA synthesizing cells (cyan) and cell nuclei (magenta)
Credit: Jackson Griffiths/Monaghan Lab
Axolotl Limb Regeneration
Instrument: Leica compound scope
Technique: FISH for dividing cells (green), nuclei (blue), and nerves (red)
Credit: Johanna Farkas/Monaghan Lab
Bovine Pulmonary Artery Endothelial Cell
Instrument: Zeiss LSM 880 using Airyscan
Stained for nuclei (blue), tubulin (red), and phalloidin (green)
Credit: Alex Lovely/CILS Institute
Cultured Microvascular Networks
Instrument: Zeiss LSM 8oo confocal
Formed under static and flow conditions within a 3D fibrin matrix
Credit: Max Winkelman/Dai Lab
Axolotl Limb Blastema Regeneration
Instrument: Zeiss Z1 Lightsheet
Cell nucelli (blue) and actively dividing cells (red)
Credit: Eun Jeon/Monaghan Lab
AQP4 Protein and Lectin
Instrument: Zeiss LSM 880
Double immunofluorescence of AQP4 protein (red) & lectin (green) from sham controls & head impact in rat substania nigra
Credit: Ian Harding/Ebong Lab
SARS-CoV-2 RBD & N Proteins
Instrument: Zeiss LSM 8oo confocal
In polymer walls of oxygen-generating COVID-19 cryogel-based vaccine
Credit: Colombani Thibault/Bencherif Lab
Mouse Tissue Section
Instrument: Zeiss LSM 880 with Airyscan Fast
HCR-FISH image at postnatal day 18
Credit: Rachel Pierce/Ionescu Lab
Rat Brain Tissue
Treated with a retroviral vector to stain neurons
Instrument: Zeiss Lightsheet Z1
Credit: Shansky Lab
C. Elegans FLP Neuron
Imaged by widefield, spatial light modulator, an enhanced setup developed by Chung Neuro Lab
Instrument: Zeiss LSM 800
Credit: Yao Wang/Chung Lab
Bovine Cartilage
Spontaneous calcium signaling of in-situ chondrocytes
Instrument: Zeiss LSM 880
Credit: Vineel Kondiboyina from Shefelbine Lab
Axolotl Larva
60-hour live image of tail regeneration with fluorescent ubiquitination-based cell-cycle indicator (FUCCI)
Instrument: Zeiss LSM 800
Credit: Tim Duerr/Monaghan Lab
Whole-mount Axolotl Limb
Limb in the process of regenerating at 21 dpa
Instrument: Zeiss Lightsheet Z.1
Credit: Eun Jeon/Monaghan Lab
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Interdisciplinary Science and Engineering Complex
ISEC 080, 805 Columbus Ave, Boston, MA, 02115