Featured Images

From the CILS Core Facility

Shipworm Tissue

Instrument: Zeiss LSM 880

Technique: 16S rRNA fluorescence in situ hybridization to locate the intracellular bacterial symbionts

Credit: Marvin Altamia/Distel lab, Ocean Genome Legacy of New England Biolabs, NU Marine Science Center

Mouse Limb Regeneration

Instrument: Zeiss Lightsheet Z1
Technique: FISH, FGF8 in yellow, SHH in magenta, and gremlin 1 in cyan
Credit: Evan Mun/Monaghan Lab

Axolotl Limb Regeneration

Instrument: Zeiss LSM 880
Technique: FISH –¬†Uromodulin(yellow), keratin 5 (cyan)-, Wnt5a (magenta)
Credit: Alex Lovely/Monaghan Lab

Brain Microvascular Network

Instrument: Zeiss LSM 800
Brain endothelial cells: dTomato (red). Pericytes: NG-2 (purple). Astrocytes: GFAP (green)
Credit: Max Winkelman/Dai Lab

African Clawed Frog (Xenopus Laevis) Embryo

Instrument: Zeiss Lightsheet Z1
Expressing green flourescent protein
Credit: Ashwin Lokapally/Sive Lab

Axolotl Limb Regeneration

Instrument: Zeiss Lightsheet Z1
Genetically encoded cell cycle reporter. Magenta: G1 phase cells. Yellow: S/G2/M phase cells
Credit: Eun Jeon/Monaghan Lab

Axolotl Limb Regeneration

Instrument: Zeiss Lightsheet Z1
Technique: FISH for three genes FGF8 (green), prrx1 (blue), and SHH (red)
Credit: Alex Lovely/Monaghan Lab

Shipworm Tissue in the Digestive Glands

Instrument: Zeiss LSM 880
Distribution of GH6 domain of a cellulase produced by intracellular bacterial symbiont
Credit: Marvin Altamia/Distel Lab

Spinal Cord – Axolotl

Instrument: Zeiss Lightsheet Z1
Stained with 5-ethynyl uridine (EU), RNA synthesizing cells (cyan) and cell nuclei (magenta)
Credit: Jackson Griffiths/Monaghan Lab

Axolotl Limb Regeneration

Instrument: Leica compound scope
Technique: FISH for dividing cells (green), nuclei (blue), and nerves (red)
Credit: Johanna Farkas/Monaghan Lab

Bovine Pulmonary Artery Endothelial Cell

Instrument: Zeiss LSM 880 using Airyscan
Stained for nuclei (blue), tubulin (red), and phalloidin (green)
Credit: Alex Lovely/CILS Institute

Cultured Microvascular Networks

Instrument: Zeiss LSM 8oo confocal
Formed under static and flow conditions within a 3D fibrin matrix
Credit: Max Winkelman/Dai Lab

Axolotl Limb Blastema Regeneration

Instrument: Zeiss Z1 Lightsheet
Cell nucelli (blue) and actively dividing cells (red)
Credit: Eun Jeon/Monaghan Lab

AQP4 Protein and Lectin

Instrument: Zeiss LSM 880
Double immunofluorescence of AQP4 protein (red) & lectin (green) from sham controls & head impact in rat substania nigra
Credit: Ian Harding/Ebong Lab

SARS-CoV-2 RBD & N Proteins

Instrument: Zeiss LSM 8oo confocal
In polymer walls of oxygen-generating COVID-19 cryogel-based vaccine
Credit: Colombani Thibault/Bencherif Lab

Mouse Tissue Section

Instrument: Zeiss LSM 880 with Airyscan Fast
HCR-FISH image at postnatal day 18
Credit: Rachel Pierce/Ionescu Lab

Rat Brain Tissue

Treated with a retroviral vector to stain neurons
Instrument: Zeiss Lightsheet Z1
Credit: Shansky Lab

C. Elegans FLP Neuron

Imaged by widefield, spatial light modulator, an enhanced setup developed by Chung Neuro Lab
Instrument: Zeiss LSM 800
Credit: Yao Wang/Chung Lab

Bovine Cartilage

Spontaneous calcium signaling of in-situ chondrocytes
Instrument: Zeiss LSM 880
Credit: Vineel Kondiboyina from Shefelbine Lab

Axolotl Larva

60-hour live image of tail regeneration with fluorescent ubiquitination-based cell-cycle indicator (FUCCI)
Instrument: Zeiss LSM 800
Credit: Tim Duerr/Monaghan Lab

Whole-mount Axolotl Limb

Limb in the process of regenerating at 21 dpa
Instrument: Zeiss Lightsheet Z.1
Credit: Eun Jeon/Monaghan Lab

Our facility

Interdisciplinary Science and Engineering Complex

ISEC 080, 805 Columbus Ave, Boston, MA, 02115