Instrument Snapshot
This confocal laser scanning microscope’s lasers are designed for deeper imaging of fixed tissue samples and live animals with high sensitivity. Paired with Airyscan module, images are 1.7 higher in resolution and sensitivity than compared to other scopes of this type.
With a large field of view and high image-acquisition speed, the microscope is able to identify and localize molecules of interest and their interactions in space and time while minimizing the input of light.
Reach out to our core facility in Boston with any questions at cils-core@northeastern.edu.
Sample Images
Axolotl Limb Regeneration
Instrument: Zeiss LSM 880
Technique: FISH – Uromodulin(yellow), keratin 5 (cyan)-, Wnt5a (magenta)
Credit: Alex Lovely/Monaghan Lab
Mouse Tissue Section
Instrument: Zeiss LSM 880 with Airyscan Fast
HCR-FISH image at postnatal day 18
Credit: Rachel Pierce/Ionescu Lab
Bovine Pulmonary Artery Endothelial Cell
Instrument: Zeiss LSM 880 using Airyscan
Stained for nuclei (blue), tubulin (red), and phalloidin (green)
Credit: Alex Lovely/CILS Institute
Rates
Internal NU
$50/hr
External Academia
$70/hr
Industry
$90/hr
Carl Zeiss Microscopy Online Campus
Education in Microscopy and Digital Imaging
LSM 880 Operating Manual
Detailed guide to LSM 880
Nature Methods:
The Fast mode for ZEISS LSM 880 with Airyscan: high-speed confocal imaging with super-resolution and improved signal-to-noise ratio
Other Instruments
Zeiss LSM 880 NLO (Upright)
NU user: $55/hr; Academia: $75/hr; Industry: $95/hr
Laser line: 405, 458, 488, 514, 561, 594, 640 nm
NIR Laser: tunable line (680-1300 nm); fixed line -1045 nm
Objective: 10×, 20×, 20×, 40×, 20×-CLARITY
Multi-photon imaging
Spectral Unmixing
Prerequisite: Bioraft Laser Safety Training Part I and II
Zeiss LSM 880 NLO (Upright)
NU user: $55/hr; Academia: $75/hr; Industry: $95/hr
Laser line: 405, 458, 488, 514, 561, 594, 640 nm
NIR Laser: tunable line (680-1300 nm); fixed line -1045 nm
Objective: 10×, 20×, 20×, 40×, 20×-CLARITY
Multi-photon imaging, Spectral Unmixing
Prerequisite: Bioraft Laser Safety Training Part I and II
Our facility
Interdisciplinary Science and Engineering Complex
ISEC 080, 805 Columbus Ave, Boston, MA, 02115