CORE FACILITIES
NU Users: 70% off rate for nightly (6PM-9AM) and weekend/holiday usage to encourage schedule staggering
Get Started With a Free Test Session
How to Get Started
Microscopy Core Facility
ZEISS LSM 800 (Inverted)
- NU user: $35/hr; Academia: $55/hr; Industry: $75/hr
- Laser line: 405, 488, 561, 640 nm
- Objective (Free Working Distance/mm): 5×(12.5), 10×(5.2), 20×(0.55), 40×(0.25), 63× Oil(0.19)
- Fixed cell/tissue imaging,
- Image Tiling
ZEISS LSM 880 NLO (upright)
- NU user: $55/hr; Academia: $75/hr; Industry: $95/hr
- Laser line: 405, 458, 488, 514, 561, 594, 640 nm
- NIR Laser: tunable line (680-1300 nm); fixed line -1045 nm
- Objective (Free Working Distance/mm): 10×(5.2), 20×W(2.1), 20×W(1.8), 40×W(2.5), 20×-CLARITY(5.6)
- Multi-photon imaging
- Superresolution (Airyscan), Spectral Unmixing
- Prerequisite:Â SciShield Laser Safety Training Part I and II
ZEISS LSM 900 (inverted)
- COMING SOON (First Half of 2024)
- Coupled with Bruker NanoWizard 4 XP AFM
- NU user: $45/hr; Academia: $65/hr; Industry: $85/hr
- Objective (Free Working Distance/mm): 5×(12.5), 10×(5.2), 20×(0.55), 63× Oil(0.19)
- Superresolution (Airyscan 2)
ZEISS LSM 880 (Inverted)
- NU user: $55/hr; Academia: $75/hr; Industry: $95/hr
- Laser line: 405, 458, 488, 514, 561, 594, 640 nm
- Objective(Free Working Distance/mm): 10×(5.2), 20×(0.55), 40×W(0.28), 40×Oil(0.13), 63×Oil(0.19)
- Cage incubator, CO2/O2 control,
- Superresolution (Airyscan), Spectral Unmixing
ZEISS LIGHTSHEET Z1
- NU user: $55/hr; Academia: $75/hr; Industry: $95/hr
- Laser line: 405, 488, 561, 638 nm
- Objective (Free Working Distance/mm): 2.5×(8.7), 5×(18.5), 10×W(3.7), 20×-CLARITY(5.6)
- CO2/temperature control,
- Large scale 3D tissue imaging
- arivis Vision4D software
ZEISS AXIO OBSERVER (DEMO, inverted)
- NU user: free; Academia: $30/hr; Industry: $50/hr
- Epifluorescence: DAPI, GFP, DsRed, Cy5
- Objective (Free Working Distance): 10×(5.2), 20×(0.55), 40×(0.13), 63×(0.19), 100×(0.17)
- Immunofluorescence labeled tissue or cell culture imaging
Bruker Nanowizard 4 xp (AFM)
- COMING SOON (First Half of 2024)
- Coupled with Zeiss LSM 900
- NU user: $45/hr; Academia: $65/hr; Industry: $85/hr
- Collrelative microsscopy: integrate with confocal
- Large scan field of 100 × 100 × 15 µm3and atomic lattice resolution
Flow Cytometry Core Facility
BECKMAN COULTER CYTOFLEX SRT CELL SORTER
- NU user: $65/hr; Academia: $110/hr; Industry: $165/hr
- 4 laser lines: 405, 488, 561, 638 nm
- 15 color detection
- 100 um nozzle
- Sort into 1-4 tubes, slides, or into various well plates
- Mixed Mode sorting
- Violet side scatter channel for small particle detection
BECKMAN COULTER CYTOFLEX S FLOW CYTOMETER
- NU user: $40/hr; Academia: $70/hr; Industry: $130/hr
- 4 laser lines: 405, 488, 561, 638 nm
- 13 color detection
- Can load tube or various well plates for analysis
- Violet side scatter channel for small particle detection
- Able to interface experiment design into SRT sorter
Preclinical Imaging Core Facility
BRUKER BIOSPEC 3T MRI
- NU user: $30/hr; Academia: $150/hr; Industry: $200/hr
- Resolution below 200 μm
- Automated animal positioning
- Multimodal rat/mouse cradle
- Two body volume coils for rat/mouse
- SAII 1030 monitoring/gating module
- Localized MR Spectroscopy
- Prerequisite: SciShield High Magnetic Fields Safety Training
VisualSonics Vevo F2 Ultrasound
- NU user: $60/hr; Academia: $110/hr; Industry: $140/hr
-
Linear Probe: 57-25 MHz for mouse cardiovascular, abdominal, reproductive; embryology; small rat vascular
-
Linear Probe: 29-15 MHz for rat cardiac & abdominal (<250g)
-
Phased Probe: 8-4 MHz for large animal cardiology & abdominal
- Prerequisite: SciShield Biosafety Training
Photon etc IR vivo NIR-II IMager
- NU user: free;
- Training: Contact Professor Allison Dennis
- Illuminaton: Laser at 670, 760, 808 nm
- Emission Range: 900-1600 nm
- Lens: 50mm f/1.4
- FOV: 5 x 4 to 15.6 x 12.5 cm
- Heated Stage
PRECISION X-RAD 320 Irradiator/Imager
- NU User: $50/h; Academia: $80/h; Industry: $110/h
- Real-time specimen viewing
- Co-registration of bioluminescence and x-ray images
- Radiation dosage control through programmable shelf
- Beam conditioning filters
- Prerequisite: Scishield X-Ray Safety Training
Additional Instruments
NanoTemper Monolith NT.Automated
NU user: free
- Step-by-Step Guide for New NU User
- Fast Kd measurement:Â ~10 min
- Low sample volume
- Wide Kd range from nM to mM range
- No need for immobilization
- Measurements in complex environments
BIO-RAD ChemiDocâ„¢ MP
NU user: $50/lab/quarter; External: $100/quarter
- Step-by-Step Guide for New NU User
- Imaging gels and blots
- UV Tray: EtBr, RGB/far red/NIR fluorescence, SYBR stain
- White Tray: Coomassie blue, copper, silver, zinc stains
- Blue Tray: GelGreen, GelStar, Fluorescein
- Image Lab Software: Windows, Mac;Â User Guide, Videos
- Wetern Blot Normalization: Guide, Video
Hourly Rates
| Instrument | NU User | Academia | Industry |
|---|---|---|---|
| Zeiss LSM 800 | $35 | $55 | $75 |
| Zeiss LSM 880 | $55 | $75 | $95 |
| Zeiss LSM 880 NLO | $55 | $75 | $95 |
| Zeiss Lightsheet Z1 | $55 | $75 | $95 |
| Zeiss Axiozoom V16 | $25 | $35 | $50 |
| Zeiss Axio Observer (Demo) | Free | $30 | $50 |
| Zeiss LSM 900 | $45 | $65 | $85 |
| Bruker NanoWizard 4 XP AFM | $45 | $65 | $85 |
| Beckman Coulter CytoFlex SRT Cell Sorter | $65 | $110 | $165 |
| Beckman Coulter CytoFlex S Flow Cytometer | $40 | $70 | $130 |
| Bruker BioSpec 3T MRI | $30 | $150 | $200 |
| Visualsonics Vevo F2 Ultrasound | $60 | $110 | $140 |
| Precision X-Rad 320 X-Ray Irradiator/Imager | $50 | $80 | $110 |
Rates listed above are for unassisted session. Hourly rate for training and assisted session is base rate+$30
NU user off-hours usage: 70%Â off from 6pm-9am on weekdays, full day on weekends/holidays
Reviewed and approved by NURes Finance (Approval Letter).
We are studying the dynamics of matrix assembly by human fibroblasts as they are producing tissue. The CILS facilities, particularly the confocal microscope with live imaging capabilities, has been indispensable to us. The team there has been wholly supportive and has enabled us to procure data we only dreamed about having previously. In fact, less than a week ago, I put fresh images from CILS directly into an NIH R01 proposal just in time for submission. It was the key missing piece of preliminary data I needed. So, thank you, CILS!
Our facility
Interdisciplinary Science and Engineering Complex
ISEC 080, 805 Columbus Ave, Boston, MA, 02115